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Antibodies sure to the blocking peptide no more bind for the epitope on the focus on protein. By evaluating the staining with the blocked antibody compared to the antibody by itself, one can see which staining is unique.
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This information outlines further attainable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
This information outlines even more probable optimization of cellular and immunoassay parameters to ensure the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide ordinarily consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the really conserved cyclin family members, whose users are characterised by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which add on the temporal coordination of every mitotic event. Cyclin E sorts a complex with and features as being a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by S stage.
Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore may contribute to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important function in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two added splice variants were being described but in-depth nucleotide sequence information and facts isn't however accessible.
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